This myopathy can be mimicked in wild-type zebrafish by the application of the AChE inhibitor galanthamin (Behra et al., 2004). The very same features enable the screening of bioactive compounds that influence complex cellular behaviors in an intact vertebrate organism. This SAR study on zebrafish embryos enabled the simultaneous evaluation of on-target and off-target effects, as well as nonspecific lethal effects, of 63 structural variants in parallel. Zebrafish can be grown easily, maintained in lab … The zebrafish embryo offers an inexpensive system that combines many features that are desirable for the development of new approaches to drug development (Bowman and Zon, 2010). The embryo begins as a yolk with a single enormous cell on top (see image, 0 h panel), which divides into two (0.75 h panel) and continues dividing until there are thousands of small cells (3.25 h panel). Most proteins have multiple functions and/or are structurally related members of protein families. It remains to be determined whether drug candidates identified using such a system will make it into the clinic at higher frequency than those selected using currently used assays. We are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide. These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. More recently, the zebrafish embryo has been applied to identify off-target effects of drug candidates. Davidson and colleagues observed a different effect when they treated embryos with pifithrin-α (PFT-α), a pharmacological inhibitor of p53. In addition, compounds can interact with multiple, even structurally unrelated, target molecules. p53 can act as a tumor suppressor and is mutated in 50% of human tumors (Hollstein et al., 1991). Als Wirbeltier besitzt der Zebrafisch viele Gene, die bei Säugetieren und damit auch beim Menschen dieselben oder ähnliche Funktionen haben. BACKGROUND: A zebrafish (Danio rerio ) teratogenicity assay has been developed and evaluated for its ability to predict the teratogenic potential of chemicals.METHODS: Zebrafish embryos were dechorionated and then exposed to a test solution from 4–6 hours post‐fertilization, and embryos or larvae were assessed up to 5 days post‐fertilization (dpf) for viability and morphology. The detailed catalog of existing phenotypic descriptions of cloned zebrafish mutants or morphants (morpholino knockdown animals) can guide the identification of the molecular nature of secondary targets (www.zebrafish.org and www.zfin.org). Zebrafisch m, Zebrabärbling, Danio rerio, E zebrafish, ein kleiner, tropischer Knochenfisch ( siehe Abb. The zebrafish as a supplement to other model organisms. This strategy will allow for the screening of compounds that affect complex cellular behaviors – for example, that of stem cells in their native environment – and will simultaneously reveal information about general toxicity and off-target effects. Please don’t hesitate to contact the Editorial Office if you have any questions or concerns. The current application round closes on 8 February 2021 – find out more. Thank you for your interest in spreading the word on Disease Models & Mechanisms. It was suggested previously that physostigmine interacts not only with AChE but also antagonizes ACh receptor (Kawai et al., 1999). Such a catalog of phenotypes, combined with the experimental advantages of the zebrafish embryo, has a high potential to improve the drug screening process: in addition to information gained through in vitro interaction studies with isolated target molecules or through biochemical analyses of cultured cells, assays carried out in zebrafish embryos will provide primary readout information regarding a chemical’s mode of action in an intact animal. Collectively, this work demonstrates that zebrafish embryos can be used to screen systematically for complex drug effects. The transparency, rapid extra-uterine development and small size of the zebrafish embryo are important characteristics that have made this organism so useful for genetic screens. The Editors of all The Company of Biologists’ journals have been considering ways in which we can alleviate concerns that members of our community may have around publishing activities during this time. We do not capture any email address. 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